Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years
نویسندگان
چکیده
منابع مشابه
Identification of Leishmania Species Using PCR Assay on Giemsa-Stained Slides Prepared From Cutaneous Leishmaniasis Patients
BACKGROUND Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. The slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملDiagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP
Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique. Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a ligh...
متن کاملidentification of leishmania species using pcr assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients.
background: leishmaniasis is a group of diseases that are created by intracellular parasites of leishmania. cutaneous leishmaniasis is considered as one of the health problems in some provinces of iran. methods: in this study, a total of 178 giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. the slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملdiagnosis and characterization of leishmania species in giemsa-stained slides by pcr-rflp
background: direct identification of leishmania species in giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. this study was designed to isolate leishmani spp. from giemsa-stained smears and to characterize them by pcr technique. methods: a total of 48 giemsa-stained slides from confirmed cases of leishmaniasis were examined under a ligh...
متن کاملDNA extraction and amplification of leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous Leishmaniasis by PCR.
DNA was isolated from 92 Giemsa-stained smears of lesions from suspected cases of cutaneous leishmaniasis and used for PCR-based diagnosis of Leishmania infection. Each smear had been examined under a light microscope at x 1,000 and scored for amastigote numbers. Although the smears had been stored for up to 4 years, all the microscopy-positive slides were also positive by PCR and four of the 1...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2006
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2006.01422.x